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Optimization of Amylase and Protease Production from Aspergillus awamori in Single Bioreactor Through EVOP Factorial Design Technique

Sangeeta Negi ; Agriculture and Food Engineering Department, Microbial Biotechnology and Downstream Processing Laboratory, IIT Kharagpur, West Bengal 721302, India
Rintu Banerjee ; Agriculture and Food Engineering Department, Microbial Biotechnology and Downstream Processing Laboratory, IIT Kharagpur, West Bengal 721302, India


Puni tekst: hrvatski pdf 28 Kb

str. 257-261

preuzimanja: 240

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Puni tekst: engleski pdf 112 Kb

str. 257-261

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Sažetak

Evolutionary operation (EVOP) factorial design technique was explored in order to economically produce amylase and protease at their optimum level in a single bioreactor by modified solid-state fermentation. Maximum yields of amylase and protease were achieved, using wheat bran as a substrate by a highly potent, locally isolated strain of Aspergillus awamori: Nakazawa MTCC 6652. The strain had been induced previously, inferring the ability to produce both enzymes concomitantly in a single bioreactor with their maximum capacity. The highest secretion of amylase and protease were measured to be 9420.6 and 1930 U/g, respectively, at 37 °C. pH and relative humidity were found to be optimum at 4 and 85 %, evaluated through EVOP method.

Ključne riječi

amylase; protease; SSF; Aspergillus awamori; EVOP; production

Hrčak ID:

109888

URI

https://hrcak.srce.hr/109888

Datum izdavanja:

15.6.2006.

Podaci na drugim jezicima: hrvatski

Posjeta: 2.395 *