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Alternative Carbon Sources from Sugar Cane Process for Submerged Cultivation of Cunninghamella bertholletiae to Produce Chitosan

Rosa Valéria da Silva Amorim ; Departamento de Biologia Molecular/Centro de Ciências Exata e da Natureza – CCEN, Universidade Federal da Paraíba – UFPB, Campus I – Cidade Universitária, 58.051-900, João Pessoa, PB, Brazil
Rodrigo Pinto Pedrosa ; Departamento de Biologia Molecular/Centro de Ciências Exata e da Natureza – CCEN, Universidade Federal da Paraíba – UFPB, Campus I – Cidade Universitária, 58.051-900, João Pessoa, PB, Brazil
Kazutaka Fukushima ; Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan
Cosme Rafael Martínez ; Departamento de Biologia Molecular/Centro de Ciências Exata e da Natureza – CCEN, Universidade Federal da Paraíba – UFPB, Campus I – Cidade Universitária, 58.051-900, João Pessoa, PB, Brazil
William MacDonald Ledingham ; Departamento de Biologia Molecular/Centro de Ciências Exata e da Natureza – CCEN, Universidade Federal da Paraíba – UFPB, Campus I – Cidade Universitária, 58.051-900, João Pessoa, PB, Brazil
Galba Maria de Campos-Takaki ; Núcleo de Pesquisas em Ciências Ambientais, Universidade Católica de Pernambuco – UNICAP, Recife, PE, Brazil


Puni tekst: hrvatski pdf 34 Kb

str. 519-523

preuzimanja: 189

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Puni tekst: engleski pdf 127 Kb

str. 519-523

preuzimanja: 650

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Sažetak

A mucoralean strain of Cunninghamella bertholletiae was used to evaluate the influence of culture medium on chitosan production. In the traditional medium for the growth of mucoralean strains, constituted of yeast extract, peptone, and D-glucose as carbon source, the highest chitosan yield found was 55 mg/g of dry mycelia in a 72-hour submerged culture. Regional substrates from sugar cane process in Northeast Brazil, as sugar cane juice and molasses, which were supplemented with 0.3 % yeast extract, were used as economic substrates to produce chitosan. The optimal production of chitosan was found in sugar cane juice medium, yielding 128 mg/g of dry mycelia in batch flasks at 28 °C. This condition did not need high concentration of sugar cane and gave a good yield of chitosan produced within 48 h (580 mg per L of medium). Molasses did not show to be a good carbon source for chitosan production.

Ključne riječi

chitosan; Cunninghamella bertholletiae; submerged culture; sugar cane juice; molasses

Hrčak ID:

110189

URI

https://hrcak.srce.hr/110189

Datum izdavanja:

15.12.2006.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.312 *