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Cultivation of CCO cells on non-porous microcarriers in low-serum conditions.

Igor Slivac ; Laboratorij za tehnologiju i primjenu stanica i biotransformacije, Prehrambeno-biotehnološki fakultet, Pierottijeva 6, 10000 Zagreb, Hrvatska
Kristina Radošević ; Laboratorij za tehnologiju i primjenu stanica i biotransformacije, Prehrambeno-biotehnološki fakultet, Pierottijeva 6, 10000 Zagreb, Hrvatska
Bogdanka Dukić ; Laboratorij za tehnologiju i primjenu stanica i biotransformacije, Prehrambeno-biotehnološki fakultet, Pierottijeva 6, 10000 Zagreb, Hrvatska
Višnja Gaurina Srček orcid.org/0000-0001-6591-3810 ; Laboratorij za tehnologiju i primjenu stanica i biotransformacije, Prehrambeno-biotehnološki fakultet, Pierottijeva 6, 10000 Zagreb, Hrvatska

Sažetak

In this study Cytodex 1 microcarriers were used for Channel Catfish Ovary (CCO) cell cultivation and biomass production in Spinner-flask.
Cell attachment, cell number and glucose consumption were determined during 312 h cultivation in two media with 5% and 2% of serum. CCO
cell cultivation in medium with lower serum concentration resulted in higher cell yield and specific growth rate when compared to standard
growth medium. Obtained results showed that tested UltraCulture medium was good choice when cultivation of CCO cells in reduced serum
conditions is demanded.

Ključne riječi

CCO cells; Cell-spin; cell yield; microcarriers; serum

Hrčak ID:

126094

URI

https://hrcak.srce.hr/126094

Datum izdavanja:

31.7.2014.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.553 *