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IMPROVED FERTILIZATION RATE AND A BIRTH AFTER INTRACYTOPLASMIC SPERM INJECTION OF TOTALLY IMMOTILE SPERM, PRESELECTED BY THE HYPO-OSMOTIC SWELLING TEST
Mojca Čižek Sajko
Borut Kovačič
Veljko Vlaisavljević
Sažetak
In vitro fertilization procedures can be applied to aid infertile couples with complete asthenozoospermia, i.e. with absence of even a single motile sperm in ejaculate. However, the probability of conception with intracytoplasmic injection of sperm (ICSI), selected at random from an immotile sperm sample, is quite slight. In couples, in which the male partner’s ejaculate contained only immotile sperm, the hypo-osmotic swelling (HOS) test was used to select viable sperm for the ICSI procedure. Material and methods. Complete asthenozoospermia as the major cause of infertility was diagnosed in 4 couples, of which two male partners had a special form of complete immotility, a sperm stump defect. Out of the total of 10 hormone-stimulated ICSI cycles, in 6 cycles the sperm for ICSI were chosen randomly, without a HOS test. In 4 cycles, the washed semen samples were incubated in a hypo-osmotic solution and only HOS-positive sperm (i.e. sperm with swollen tail) were used for ICSI. Results. Of 6 ICSI cycles without selection of viable sperm using the HOS test, total fertilization failure was noted in 3. Only a total of 20.7% (6/29) oocytes were fertilized. In all 4 ICSI cycles with performed HOS test prior to sperm injection, embryos for transfer were obtained. An 81.8% (18/22) fertilization rate was registered and one twin pregnancy which resulted in a birth of a healthy singleton. Conclusion. We can confirm that also in cases of complete asthenozoospermia, even in those, combined with stump defect, good fertilization rate and pregnancies can be achieved after selecting the viable sperm by HOS test prior to the ICSI procedure.
Ključne riječi
complete asthenozoospermia; ICSI; HOS test; pregnancy
Hrčak ID:
15598
URI
Datum izdavanja:
1.12.2004.
Posjeta: 3.014 *