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Food Technology and Biotechnology, Vol. 54 No. 4, 2016.

Prethodno priopćenje

https://doi.org/10.17113/ftb.54.04.16.4647

Prevalence of Thermotolerant Campylobacter spp. in Chicken Meat in Croatia and Multilocus Sequence Typing of a Small Subset of Campylobacter jejuni and Campylobacter coli Isolates

Marina Mikulić ; Hrvatski veterinarski institut, Savska cesta 143, HR-10000 Zagreb, Hrvatska
Andrea Humski ; Hrvatski veterinarski institut, Savska cesta 143, HR-10000 Zagreb, Hrvatska
Bela Njari ; Sveučilište u Zagrebu, Veterinarski fakultet, Heinzelova 55, 10000 Zagreb, Hrvatska
Mario Ostović ; Sveučilište u Zagrebu, Veterinarski fakultet, Heinzelova 55, 10000 Zagreb, Hrvatska
Sanja Duvnjak ; Sveučilište u Zagrebu, Veterinarski fakultet, Heinzelova 55, 10000 Zagreb, Hrvatska
Željko Cvetnić ; Sveučilište u Zagrebu, Veterinarski fakultet, Heinzelova 55, 10000 Zagreb, Hrvatska

Puni tekst: engleski pdf 405 Kb

str. 475-481

preuzimanja: 628

citiraj

Sažetak

In order to detect thermotolerant Campylobacter spp., 241 samples of fresh chicken meat, at retail in Croatia, were analysed according to a standard method, followed by biochemical test and molecular polymerase chain reaction/restriction enzyme analysis for exact species determination. Campylobacter spp. prevalence was 73.86 %. Campylobacter jejuni and Campylobacter coli were isolated from 53.53 and 15.35 % of the samples, respectively. In 4.98 % of isolates thermotolerant Campylobacter spp. were not determined. The multi locus sequence typing method was used to evaluate genetic diversity of eight Campylobacter jejuni and four Campylobacter coli isolates. To our knowledge, these results of genotyping provided the first data on the presence of sequence types (STs) and clonal complexes (CCs) of Campylobacter jejuni and C. coli isolates in Croatia. By applying the multilocus sequence typing, a new allele of tkt gene locus was discovered and marked tkt508. The C. jejuni ST 6182 and C. coli ST 6183 genotypes were described for the fi rst time, and all other identified genotypes were clustered in the previously described sequence types and clonal complexes. These findings provide useful information on the prevalence and epidemiology of Campylobacter jejuni and C. coli in Croatia.

Ključne riječi

thermotolerant Campylobacter; chicken meat in Croatia; prevalence; multilocus sequence typing (MLST) method

Hrčak ID:

170004

URI

https://hrcak.srce.hr/170004

Datum izdavanja:

2.12.2016.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.769 *