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STABILITY OF 10-HDA IN LYOPHILIZED ROYAL JELLY AND IN THE FINISHED PRODUCTS

Martin Lalić ; Apipharma d.o.o., Pharmaceutical-food, cosmetic industry and trade, Jeronima Kavanjina 26, 10090 Zagreb, Croatia
Ana Soldić orcid id orcid.org/0000-0003-3230-9216 ; Apipharma d.o.o., Pharmaceutical-food, cosmetic industry and trade, Jeronima Kavanjina 26, 10090 Zagreb, Croatia
Andrija Lalić ; Apipharma d.o.o., Pharmaceutical-food, cosmetic industry and trade, Jeronima Kavanjina 26, 10090 Zagreb, Croatia
Zdenka Lalić ; Apipharma d.o.o., Pharmaceutical-food, cosmetic industry and trade, Jeronima Kavanjina 26, 10090 Zagreb, Croatia


Puni tekst: engleski pdf 505 Kb

str. 69-73

preuzimanja: 430

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Sažetak

This paper aims to examine the stability of lyophilized royal jelly by monitoring the content of its biologically active component, 10-hydroxy-2-decenoic acid (10-HDA). Stability was monitored in the lyophilized royal jelly and in the finished products containing it. Analyses were performed using high performance liquid chromatography (HPLC) with diode array detector. Chromatographic conditions for HPLC with diode array detection were as followed: InterSustain® C18 column, 150 x 40 mm, 5 μm; column temperature, 40 °C; mobile phase was a mixture of methanol: ultrapure water: phosphoric acid (250: 250: 1,25) with a flow rate of 1 mL/min. The detection wavelength was UV 210 nm. The amount of 10-HDA decreased regardless of storage conditions (room temperature and light protection), but the content of 10-HDA in any sample of the lyophilized royal jelly did not decline below the declared 3.5% during the shelf life. During the monitoring period, the analysed finished products also shown a decrease in the amount of 10-HDA, but if stored under recommended conditions, all analysed products contain the amount of 10-HDA in accordance with the declared value.

Ključne riječi

10-HDA; lyophilized royal jelly; stability

Hrčak ID:

261761

URI

https://hrcak.srce.hr/261761

Datum izdavanja:

31.12.2020.

Posjeta: 801 *