Izvorni znanstveni članak
Production of Anti-Complementary Exopolysaccharides from Submerged Culture of Flammulina velutipes
Kwang-Soon Shin
; Department of Food Science and Biotechnology, Kyonggi University, Suwon, Kyonggi-do 443–760, Korea
Kwang-Won Yu
; Divison of Food and Biotechnology, Chungju National University, Jeungpyeong-gun, Chungbuk 368–701, Korea
Hyun-Kyung Lee
; Department of Food Science and Biotechnology, Kyonggi University, Suwon, Kyonggi-do 443–760, Korea
Ho Lee
; Department of Food Science and Biotechnology, Kyonggi University, Suwon, Kyonggi-do 443–760, Korea
Won-Dai Cho
; Agricultural Cooperative College, Kyonggi 142–7070, Korea
Hyung-Joo Suh
; Department of Food and Nutrition, Korea University, 1 Jeongneung-dong, Sungbuk-ku, Seoul 136–703, Korea
Sažetak
Seven species of basidiomycetes have been investigated for anti-complementary activity in hot water extracts and ethanol soluble fractions. Since Flammulina velutipes had the most potent activity, culture conditions for its mycelial growth were optimized to increase the production efficiency of anti-complementary exopolysaccharides. The optimal medium composition was (in g/L): galactose 15, sodium nitrate 5, glutamic acid 3, KH2PO4 2.5 and MgSO4·7H2O 0.6. Optimal production of anti-complementary activity was achieved at pH=3.5–5.5 and 25 °C. With these optimal medium and culture conditions, mycelial dry mass was maximized at 3.17 mg/mL after 6 days of cultivation in a 5-liter stirred-tank bioreactor, without pH control. The anti-complementary activity of exopolysaccharides increased sharply after 4 days of cultivation, and showed a high level at 5–6 days of cultivation. A periodate-labile carbohydrate moiety played a leading role in the anti-complementary activity exhibited by exopolysaccharide produced from F. velutipes. Results of tests on the anti-complementary activity in the absence of Ca²+ and immunoelectrophoresis indicated that the mode of complement activation by exopolysaccharide from F. velutipes is via both the classical and alternative pathways and that the activation degree is almost the same in each pathway.
Ključne riječi
anti-complementary; exopolysaccharides; submerged culture; bioreactor; Flammulina velutipes
Hrčak ID:
24179
URI
Datum izdavanja:
14.9.2007.
Posjeta: 1.935 *