Kratko priopćenje
Production and Characterization of Poly(3-Hydroxybutyrate) from Oleic Acid by Ralstonia eutropha
Vitor Henrique Grigull
; Laboratory of Biotechnology, University of Joinville Region, Santa Catarina, BR-89201-972 Brazil
Delne Domingos da Silva
; Laboratory of Biotechnology, University of Joinville Region, Santa Catarina, BR-89201-972 Brazil
Michele Cristina Formolo Garcia
; Laboratory of Biotechnology, University of Joinville Region, Santa Catarina, BR-89201-972 Brazil
Sandra Aparecida Furlan
; Laboratory of Biotechnology, University of Joinville Region, Santa Catarina, BR-89201-972 Brazil
Ana Paula Testa Pezzin
; Laboratory of Biotechnology, University of Joinville Region, Santa Catarina, BR-89201-972 Brazil
Andréa Lima dos Santos Schneider
; Laboratory of Biotechnology, University of Joinville Region, Santa Catarina, BR-89201-972 Brazil
Gláucia Falcão Aragão
; Federal University of Santa Catarina, Florianópolis, BR-88800-000 Brazil
Sažetak
The aim of this research is to investigate the influence of oleic acid concentration on the cell growth and the physical properties of the polymer formed by cultures of Ralstonia eutropha in mineral medium. Cells were cultivated in Erlenmeyer flasks with 300 mL of mineral medium, containing glucose and fructose as a carbon source (30 g/L) and ammonium sulphate (5.0 g/L) as a nitrogen source. Oleic acid was added as nutritional supplement in different concentrations (0, 0.3, 0.9, 1.5 and 3.0 g/L) and the cells were incubated at 30 °C and 150 rpm. The films prepared by casting were evaluated by X-ray diffraction, thermogravimetry and differential scanning calorimetry. These results indicate that the increase of oleic acid concentrations leads to a higher specific growth rate and cell productivity. The characterization of the films revealed that the increase of the concentration of oleic acid from 0 to 1.5 g/L has no influence on thermal behaviour and crystallinity degree. However, the thermal stability, melting temperature, glass transition temperatures and crystallinity degree decreased when 3.0 g/L of oleic acid were used.
Ključne riječi
biopolymers; Ralstonia eutropha; oleic acid; characterization
Hrčak ID:
24414
URI
Datum izdavanja:
13.6.2008.
Posjeta: 2.362 *