Izvorni znanstveni članak

https://doi.org/10.24099/vet.arhiv.2406

Molecular detection of Cryptosporidium and Giardia duodenalis in canine faecal samples in the Qinghai Tibetan Plateau area, China

Yingna Jian ; Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, China
Xueyong Zhang ; Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, China *
Liqing Ma ; Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, China
Guanghua Wang ; Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, China
Qigang Cai ; Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, China
Geping Wang ; Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, China
Xiuping Li ; Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, China
Panagiotis Karanis ; Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, China; Medical Faculty and University Hospital Cologne, University of Cologne, Cologne, Germany; University of Nicosia Medical School, Department of Basic and Clinical Sciences, Anatomy Centre, Nicosia, Cyprus

* Dopisni autor.

Sažetak

Dogs act as natural reservoirs of a large number of zoonotic pathogens, including the intestinal Cryptosporidium spp. and Giardia duodenalis, the most relevant protozoan species causing gastrointestinal diseases worldwide. An epidemiological study aiming to assess the prevalence and molecular diversity of Cryptosporidium and G. duodenalis was conducted in the Qinghai Tibetan Plateau area (QTPA), Northwest China. A total of 217 dog faecal samples were collected from sheep farms, dog farms and pet hospitals. The species/genotypes of Cryptosporidium and G. duodenalis isolated from dogs were identified by the PCR-based method targeting the partial 18S ribosomal RNA gene for Cryptosporidium and β-giardin gene for G. duodenalis. The results of Cryptosporidium spp. and G. duodenalis infections in dogs showed an overall prevalence of 2.8% (6/217) and 6.9% (15/217), respectively. No Cryptosporidium and G. duodenalis co-infections were observed. The PCR-sequence results confirmed detection of C. parvum (n=3), C. canis (n=2), C. andersoni (n=1) and G. duodenalis assemblages B (n=9), C (n=3) and D (n=3) in dogs from the QTPA. The results of the present study demonstrated that dogs in the QTPA are commonly exposed to Cryptosporidium spp. and G. duodenalis. This may indicate that dogs are potential sources of infection of Cryptosporidium spp. and G. duodenalis transmitting to humans and animals.

Ključne riječi

Cryptosporidium; Giardia duodenalis; dogs; prevalence; molecular detection; Qinghai Tibetan Plateau Area

Hrčak ID:

330428

URI

https://hrcak.srce.hr/330428

Datum izdavanja:

1.4.2025.

Podaci na drugim jezicima: hrvatski

Posjeta: 934 *