Original scientific paper

https://doi.org/10.24099/vet.arhiv.2406

Molekularna detekcija parazita Cryptosporidium i Giardia duodenalis u uzorcima fecesa pasa s tibetanske Qinghai visoravni u Kini

Yingna Jian ; Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, China
Xueyong Zhang ; Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, China *
Liqing Ma ; Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, China
Guanghua Wang ; Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, China
Qigang Cai ; Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, China
Geping Wang ; Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, China
Xiuping Li ; Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, China
Panagiotis Karanis ; Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, China; Medical Faculty and University Hospital Cologne, University of Cologne, Cologne, Germany; University of Nicosia Medical School, Department of Basic and Clinical Sciences, Anatomy Centre, Nicosia, Cyprus

* Corresponding author.

Abstract

Psi su prirodni rezervoari velikog broja zoonotskih patogena, uključujući intestinalne parazite Cryptosporidium spp. i Giardia duodenalis, najvažnije protozoalne vrste koje uzrokuju gastrointestinalne bolesti diljem svijeta. Kako bi se ustanovila raširenost i molekularna raznolikost parazita Cryptosporidium i G. duodenalis, provedeno je epidemiološko istraživanje u području tibetanske Qinghai visoravni (QTPA) u sjevernoj Kini. Prikupljeno je ukupno 217 uzoraka fecesa pasa s farmi ovaca, iz uzgajališta pasa i iz bolnica za kućne ljubimce. Izolirane vrste odnosno genotipovi parazita Cryptosporidium i G. duodenalis identificirani su PCR-om usmjerenim na dio gena za ribosomsku RNA 18S za Cryptosporidium i gena β-giardin za G. duodenalis. Rezultati invazije parazitima Cryptosporidium spp. i G. duodenalis u pasa pokazali su ukupnu prevalenciju od 2,8% (6/217) za Cryptosporidium spp. i 6,9% (15/217) za G. duodenalis. Nije uočena istodobna invazija ovim dvama parazitima. Sekvenciranje PCR-om potvrdilo je nalaz C. parvum (n=3), C. canis (n=2), C. andersoni (n=1) i G. duodenalis podtipova B (n=9), C (n=3) i D (n=3). Rezultati su pokazali da su psi na istraživanom području često izloženi parazitima Cryptosporidium spp. i G. duodenalis. Navedeno potvrđuje da bi psi mogli biti potencijalan izvor invazije parazitima Cryptosporidium spp. i G. duodenalis za ljude i druge vrste životinja.

Keywords

Cryptosporidium; Giardia duodenalis; psi; prevalencija; molekularna detekcija; tibetanska visoravan

Hrčak ID:

330428

URI

https://hrcak.srce.hr/330428

Publication date:

1.4.2025.

Article data in other languages: english

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