Izvorni znanstveni članak
Phenolic compounds and minerals in the leaves of olive cultivar 'Leccino': effect of sampling time
Simone Bernobić
orcid.org/0009-0009-3721-2131
; Institut za poljoprivredu i turizam, Karla Huguesa 8, Poreč, Hrvatska
*
Dean Ban
; Institut za poljoprivredu i turizam, Karla Huguesa 8, Poreč, Hrvatska
Marija Polić Pasković
; Institut za poljoprivredu i turizam, Karla Huguesa 8, Poreč, Hrvatska
Smiljana Goreta Ban
; Institut za poljoprivredu i turizam, Karla Huguesa 8, Poreč, Hrvatska
Nikola Major
; Institut za poljoprivredu i turizam, Karla Huguesa 8, Poreč, Hrvatska
Igor Palčić
; Institut za poljoprivredu i turizam, Karla Huguesa 8, Poreč, Hrvatska
Ivan Dlačić
orcid.org/0009-0002-1808-5877
; Institut za poljoprivredu i turizam, Karla Huguesa 8, Poreč, Hrvatska
Igor Pasković
; Institut za poljoprivredu i turizam, Karla Huguesa 8, Poreč, Hrvatska
* Dopisni autor.
Sažetak
Olive (Olea europaea L.) is one of the most significant agricultural crops in the Mediterranean region. In Croatia, the 'Leccino' cultivar is the most widely introduced variety in new olive plantations. The most prominent olive product is olive oil, which is notable for its high content of monounsaturated fatty acids and various beneficial bioactive compounds, particularly phenolic compounds. In addition to olive oil and fruit, olive leaves have long been used in traditional medicine, primarily due to compounds such as oleuropein, hydroxytyrosol, tyrosol, and luteolin, known for their antioxidant, anti-inflammatory, and antitumor properties. The concentration of phenolic compounds in leaves is significantly influenced by different agroecological factors and is also correlated with nutrient concentrations within the plant. The aim of this research was to determine the impact of different sampling dates (80, 110 and 140 days after full bloom, which occurred on June 1, and during pruning) on the concentration of phenolic compounds and minerals in the leaves of the olive cultivar 'Leccino'. The study was conducted in Sikovo, Zadar County, Croatia. A total of 100 leaves per tree were sampled, each tree representing one replicate. After sampling, the leaves were washed, dried, ground, and prepared for further analyses. Phenolic compounds were quantified using HPLC after methanolic extraction, while mineral elements were analyzed using ICP-MS and FAAS. Data were statistically analyzed by one-way analysis of variance (ANOVA), followed by LSD test for significant differences and Pearson correlation (p < 0.05). The results indicated a significant effect of sampling date on concentrations of oleuropein, oleacein, oleanolic acid, and the elements P, K, Ca, Mg, Fe, Mn, S, Si, and Zn. The highest concentrations of these phenolic compounds were recorded during pruning, when the lowest potassium (K) and highest magnesium (Mg) concentrations were also observed. These results contribute to understanding the influence of the developmental stage of olive and seasonal changes on the quality and composition of olive leaves. These findings are particularly important for the nutritional and pharmaceutical application of olive leaf extracts. Monitoring the dynamics of phenolic compounds and minerals in different regions of Croatia enables the optimization of orchard management, thereby contributing to increased yields, improved olive oil quality, and preservation of the nutritional value of the leaves. Further research will focus on the same parameters in the leaves of 'Leccino' cultivar to assess the influence of different agroecological conditions and regional specificities on leaf quality and to confirm the general applicability of the results. In this way, it will be possible to develop scientifically based recommendations for sustainable olive orchard management in Croatia and beyond.
Ključne riječi
secondary metabolites; oleuropein; oleacein; oleanolic acid; nutrients
Hrčak ID:
339993
URI
Datum izdavanja:
1.10.2025.
Posjeta: 0 *