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Stručni rad

Sperm morphology assessment according to WHO and strict criteria: method comparison and intra-laboratory variability

Andrea Čipak ; Klinika za ženske bolesti i porode, Klinički bolnički centar "Zagreb", Zagreb, Hrvatska
Patrik Stanić ; Klinika za ženske bolesti i porode, Klinički bolnički centar "Zagreb", Zagreb, Hrvatska
Koraljka Đurić ; Medicinsko biokemijski laboratorij, Poliklinika "Sunce", Zagreb
Tihana Serdar ; Klinički zavod za laboratorijsku dijagnostiku, Klinička bolnica Dubrava, Zagreb, Hrvatska
Ernest Suchanek ; Fertility Clinic,Tawam Hospital, Johns Hopkins Medicine, Al Ain, Abu Dhabi, U.A.E.


Puni tekst: hrvatski pdf 172 Kb

str. 87-94

preuzimanja: 445

citiraj

Puni tekst: engleski pdf 172 Kb

str. 87-94

preuzimanja: 469

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Sažetak

Background: Assessment of sperm morphology is one of the most important steps in the evaluation of male partner in infertile couples. However, significant inter- and intra-laboratory variations can cause difficulties in interpretation, misdiagnoses, and consequently lead to confusion. Therefore, it is necessary to minimize these variations to eliminate consequential errors and ensure intra- and inter-laboratory reproducibility.
Materials and methods: Semen specimens were obtained from 49 consecutive male patients attending Andrology Laboratory for fertility evaluation. Two sperm morphology assessment criteria were compared: 1) semen smear prepared by Giemsa and assessed by World Health Organization (WHO) criteria; and 2) semen smear prepared by Spermac and assessed by strict criteria. Intra-laboratory comparison of morphological examination was also carried out.
Results: The diagnosis of teratozoospermia by both WHO and strict criteria was concordant in 45 of 49 cases. Intra-rater agreement between the observers was similar for WHO and strict criteria (kappa, 0.700 vs. 0.715).
Conclusions: Morphology assessment by WHO and strict criteria is concordant in diagnosing teratozoospermia and good inter-observer agreement can be achieved after proper training, mindful smear examination and complying with the classification systems.

Ključne riječi

spermatozoa, infertility, male, observer variation

Hrčak ID:

32268

URI

https://hrcak.srce.hr/32268

Podaci na drugim jezicima: hrvatski

Posjeta: 1.892 *