Kratko priopćenje
Culture Optimization and Amino Acid Composition of Cr-Enriched Mycelia of Pleurotus cornucopiae SD-01
Fan-Yun Meng
; College of Life Sciences, Shandong Agricultural University, Taian, Shandong, CN-271018, PR China
Xiao-Nan Liu
; College of Life Sciences, Shandong Agricultural University, Taian, Shandong, CN-271018, PR China
Le Jia
; College of Life Sciences, Shandong Agricultural University, Taian, Shandong, CN-271018, PR China
Zhen Song
; College of Life Sciences, Shandong Agricultural University, Taian, Shandong, CN-271018, PR China
Peng Deng
; College of Life Sciences, Shandong Agricultural University, Taian, Shandong, CN-271018, PR China
Ke-Ming Fan
; College of Life Sciences, Shandong Agricultural University, Taian, Shandong, CN-271018, PR China
Sažetak
Chromium(III) is an essential trace element for humans and animals. Pleutorus cornucopiae SD-01 is a nutritional and functional mushroom containing many kinds of bioactive ingredients. The aims of this work are to optimize the conditions of P. cornucopiae SD-01 cultivation with Cr enrichment in submerged culture by determining the dry cell mass, Cr content in mycelia and the rate of Cr enrichment, and to analyze the amino acid composition of Cr-enriched mycelia. The optimal medium contained (in g/L): potato 200, sucrose 25, yeast extract 4, KH2PO4 1 and MgSO4·7H2O 1. The optimum parameters of liquid culture were temperature 25 °C, cultivation time 6 days, the volume of the medium 100 mL, rotation speed 160 rpm and initial pH=6.5. Under the optimized conditions, the values of the dry cell mass, Cr content in mycelia and the rate of Cr enrichment were (6.63±0.35) g/L, (3670±211) μg/g and (12.15±1.01) % respectively, which were by (23.23±1.22), (18.19±1.06) and (45.68±2.67) % respectively, higher than those in the control. Chromium(III) in Cr-enriched mycelia was mainly combined with protein and polysaccharide. The contents of total amino acids and essential amino acids in Cr-enriched mycelia were increased by (31.25±0.58) and (44.26±0.76) %, respectively.
Ključne riječi
chromium(III); Cr-enrichment; Pleutorus cornucopiae SD-01; culture optimization; amino acid composition
Hrčak ID:
48451
URI
Datum izdavanja:
5.3.2010.
Posjeta: 1.727 *