Original scientific paper

The Preparation and Bioactivity Research of Agaro-Oligosaccharides

Hai-Min Chen ; Key Laboratory of Marine Biotechnology, Post Box 71, Ningbo University, Ningbo 315211, P. R. China
Li Zheng ; Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P. R. China
Xiao-Jun Yan ; Key Laboratory of Marine Biotechnology, Post Box 71, Ningbo University, Ningbo 315211, P. R. China

Abstract

Agaro-oligosaccharides were hydrolytically obtained from agar using hydrochloric acid, citric acid, and cationic exchange resin (solid acid). The FT-IR and NMR data showed that the hydrolysate has the structure of agaro-oligomers. Orthogonal matrix method was applied to optimize the preparation conditions based on α-naphthylamine end-labeled HPLC analysis method. The optimal way for oligosaccharides with different degree of polymerization (DP) was achieved by using solid acid degradation, which could give high yield and avoid solution neutralization process. Agaro-oligosaccharides with high purity were consequently obtained by activated carbon column isolation. Furthermore, the antioxidant and -glucosidase inhibitory activity of three fractions were also investigated. The result indicated that 8 % ethanol-eluted fraction showed highest activity against α-glucosidase with IC50 of 8.84 mg/mL, while 25 % ethanol-eluted fraction possessed excellent antioxidant ability.

Keywords

agaro-oligosaccharides; orthogonal matrix; HPLC, acid; degradation; antioxidant; α
-glucosidase

Hrčak ID:

110338

URI

https://hrcak.srce.hr/110338

Publication date:

15.3.2005.

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