Original scientific paper

Screening for Extracellular Lipase Enzymes with Transesterification Capacity in Mucoromycotina Strains

Alexandra Kotogán ; Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, HU-6726 Szeged, Hungary
Brigitta Németh ; Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, HU-6726 Szeged, Hungary
Csaba Vágvölgyi ; Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, HU-6726 Szeged, Hungary
Tamás Papp ; Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, HU-6726 Szeged, Hungary
Miklós Takó orcid.org/0000-0002-1219-1049 ; Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52, HU-6726 Szeged, Hungary

Abstract

In this study, 169 zygomycetes fungal strains including some cold-tolerant isolates were screened for their extracellular lipolytic activity towards tributyrin. Nineteen of them were outstanding in their enzyme production as they developed the largest lipolytic halo
around the colonies in plate tests. Mortierella alpina, M. echinosphaera, Mucor corticolus, Rhizomucor miehei, Rhizopus oryzae, Rh. stolonifer, Umbelopsis autotrophica, U. isabellina, U.
ramanniana var. angulispora and U. versiformis were selected for further studies to characterise their lipolytic enzyme production in detail. In these assays, effect of Tween 80 and palm, soybean, sunflower, olive, extra virgin olive, wheat germ, corn germ, sesame seed, pumpkin seed and cottonseed oils on the enzyme activities was investigated, and wheat bran-based submerged and solid-state fermentations were also tested. Tween 80 and olive oil proved to be efficient inductors for lipolytic enzyme production, which was also enhanced when wheat bran was used as support. Addition of mineral salts and olive oil to the solid fermentation medium resulted in at least 1.5-fold increment in the enzyme activities of the crude extracts. Organic synthesis was also assayed by the selected lipases, in which enzymes from the fungi R. miehei, Rh. stolonifer and M. echinosphaera gave the best yields during transesterification reactions between p-nitrophenyl palmitate and ethanol.

Keywords

zygomycetes; tributyrin; wheat bran; p-nitrophenyl palmitate; transesterification

Hrčak ID:

118553

URI

https://hrcak.srce.hr/118553

Publication date:

17.3.2014.

Article data in other languages: croatian

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