Original scientific paper

https://doi.org/10.15255/KUI.2013.027

Optimization of Total Flavonoids Extraction from Coreopsis tinctoria Nutt. by Response Surface Methodology

X. F. Liu ; School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, PR China
L. Liu ; School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, PR China
Y. G. Wang ; School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, PR China
F. F. Leng ; School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, PR China
S. W. Wang ; School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, PR China
Y. C. Li ; School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, PR China

Abstract

Response surface methodology (RSM) was applied to predict optimum conditions for extraction of flavonoid from Coreopsis tinctoria Nutt. A central composite design (CCD) was used to monitor the effect of extraction temperature, extraction time, and water-to-material ratio on yield of total flavonoids. The optimal extraction conditions were obtained as water-to-material ratio of 55 ml g−1, extraction temperature of 80 °C and extraction time of 70 minutes. Under these conditions, the average total flavonoids yield, according to the mass of raw material, was 9.0 ± 0.6 %, which corresponds to the predicted value of 8.9 %. Thus, the extraction method was applied successfully to extract total flavonoids from C. tinctoria.

Keywords

Coreopsis tinctoria Nutt.; flavonoid; reflux extraction; response surface methodology

Hrčak ID:

129289

URI

https://hrcak.srce.hr/129289

Publication date:

4.11.2014.

Article data in other languages: croatian

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