Agriculture, Vol. 21 No. 1 SUPPLEMENT, 2015.
Original scientific paper
https://doi.org/10.18047/poljo.21.1.sup.12
LACTATE DEHYDROGENASE GENE GENOTYPIZATION FOR SPECIES IDENTIFICATION IN A FISH FARM ON THE RIVER NERETVA
Polonca Margeta
; Josip Juraj Strossmayer University of Osijek, Faculty of Agriculture in Osijek, Kralja Petra Svačića 1d, 31000 Osijek, Croatia
Ivan Bogut
; Josip Juraj Strossmayer University of Osijek, Faculty of Agriculture in Osijek, Kralja Petra Svačića 1d, 31000 Osijek, Croatia
Marko Ivanković
; Federal Agromediterranean Institute Mostar, Biskupa Čule 10, 88000 Mostar, Bosnia and Herzegovina
Vladimir Margeta
; Josip Juraj Strossmayer University of Osijek, Faculty of Agriculture in Osijek, Kralja Petra Svačića 1d, 31000 Osijek, Croatia
Abstract
There are severale Salmonid species, found in the river Neretva basin, among which S. trutta and S. obtusirostris. Also, natural hybrids such as S. obtusirostris x S. trutta have been observed. In one fish farm on the river Neretva, S. trutta and S. obtusirostris were decided to breed separately. Parental fishes were separated phenotypicaly on the basis of the morphological signs. PCR-RFLP analysis of the exon 3 to exon 4 part of the lactate dehydrogenase (LDH) C1* gene with restriction endonuclease RsaI was employed to identify the presence of other species representatives or intercrosses in two groups of juvenille fishes. Using this method, we were able to identify two S. trutta representatives in the S. obtusirostris group.
Keywords
Lactate dehydrogenase gene; PCR-RFLP; S. trutta; S. obtusirostris
Hrčak ID:
150616
URI
Publication date:
2.9.2015.
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