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https://doi.org/10.18047/poljo.21.1.sup.12

LACTATE DEHYDROGENASE GENE GENOTYPIZATION FOR SPECIES IDENTIFICATION IN A FISH FARM ON THE RIVER NERETVA

Polonca Margeta ; Josip Juraj Strossmayer University of Osijek, Faculty of Agriculture in Osijek, Kralja Petra Svačića 1d, 31000 Osijek, Croatia
Ivan Bogut ; Josip Juraj Strossmayer University of Osijek, Faculty of Agriculture in Osijek, Kralja Petra Svačića 1d, 31000 Osijek, Croatia
Marko Ivanković ; Federal Agromediterranean Institute Mostar, Biskupa Čule 10, 88000 Mostar, Bosnia and Herzegovina
Vladimir Margeta ; Josip Juraj Strossmayer University of Osijek, Faculty of Agriculture in Osijek, Kralja Petra Svačića 1d, 31000 Osijek, Croatia


Puni tekst: engleski pdf 537 Kb

str. 56-58

preuzimanja: 568

citiraj


Sažetak

There are severale Salmonid species, found in the river Neretva basin, among which S. trutta and S. obtusirostris. Also, natural hybrids such as S. obtusirostris x S. trutta have been observed. In one fish farm on the river Neretva, S. trutta and S. obtusirostris were decided to breed separately. Parental fishes were separated phenotypicaly on the basis of the morphological signs. PCR-RFLP analysis of the exon 3 to exon 4 part of the lactate dehydrogenase (LDH) C1* gene with restriction endonuclease RsaI was employed to identify the presence of other species representatives or intercrosses in two groups of juvenille fishes. Using this method, we were able to identify two S. trutta representatives in the S. obtusirostris group.

Ključne riječi

Lactate dehydrogenase gene; PCR-RFLP; S. trutta; S. obtusirostris

Hrčak ID:

150616

URI

https://hrcak.srce.hr/150616

Datum izdavanja:

2.9.2015.

Posjeta: 1.199 *