Acta Botanica Croatica, Vol. 40 No. 1, 1981.
Original scientific paper
Maja Kovač
; Slovenija
Miran Vardjan
; Hrvatska
Abstract
Triglycerides are the main reserve substance of fir seeds (Abies alba Mill.). Especially, unsaturated fatty acids are present in fir seeds. Among determined fatty acids the presence of linoleic acid was particularly noted next to oleic, palmitic and stearic acids, which were found to be present in smaller amounts in stratified and non-stratified seeds at different intervals prior to and during germination. The results indicate that before germination, especially sugars are utilized although they are not the main reserve substance. The decomposition of reserve triglycerides initiates in already germinating seeds and is associated with an increase in the amount of soluble sugars.
By using incubation with radioactive glycerol, a synthesis of lipids was observed during the induction of germination. The synthesis of complex lipids was more pronounced than that of simple lipids in all measurements. By the use of autoradiography it was demonstrated that nearly all spots of complex lipids were radioactive, whereas from simple lipids only diglycerides but not triglycerides, exhibited radioactivity.
When the incorporation of radioactive glycerol into the lipid fraction, the aqueous fraction and C02 were examined, the highest radioactivity was found in C02, whereas the aqueous fraction was less radioactive and the lipid fraction exhibited the lowest radioactivity. Quantitative incorporation was similar in all three fractions in stratified as well as non-stratified seeds. However, the rate of incorporation was found to be exactly reverse.
Keywords
Hrčak ID:
158434
URI
Publication date:
31.12.1981.
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