Original scientific paper
https://doi.org/10.15567/mljekarstvo.2016.0304
Polymorphism of κ-casein and β-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia
Milan Maletić
; Department of Reproduction, Fertility and Artificial Insemination, Faculty of Veterinary Medicine, University of Belgrade, Bul. oslobođenja 18, 11000 Belgrade, Serbia
Nevenka Aleksić
; Department of Parasitology, Faculty of Veterinary Medicine, University of Belgrade, Bul. oslobođenja 18, 11000 Belgrade, Serbia
Branislav Vejnović
; Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Bul. oslobođenja 18, 11000 Belgrade, Serbia
Dragan Nikšić
; Institute for Animal Husbandry, Autoput 16, 11080 Belgrade-Zemun, Serbia
Milan Kulić
; Department for human genetics, Faculty of Medicine, University of East Sarajevo, Studentska 5, 73300 Foča, Republic of Srpska, Bosnia and Herzegovina
Božidar Đukić
; Medicines and Medical Devices Agency of Serbia, Vojvode Stepe 451, 11221 Belgrade
Dragan Ćirković
; Department of Chemical and Technological Sciences, State University of Novi Pazar, Vuka Karadžića bb, 36300 Novi Pazar, Serbia
Abstract
The aim of this study was to determine the distribution of κ-casein (κ-CN) and β-lactoglobulin (β-Lg) genotypes in the autochtonous (Busha) and dairy (Holstein-Friesian, HF) cattle breeds with PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism). For the amplification of κ-CN and β-Lg gene fragments specific primers were used. After digestion with specific endonucleases genotypes were determined for both genes in 18 Busha and 19 HF cows. The results showed that κ-CN gene was represented with the AA genotype in 31.58 % HF cows, AB in 52.63 % cows, whilst the genotype BB was found in 15.79 % cows only. Among the examined Busha cattle 44.44 % cows had AA genotype and 55.56 % genotype AB for κ CN. As for β-Lg gene in HF breed, AA genotype was found in 26.31 % cows, AB in 63.16 % and BB in 10.53 % cows. In Busha cows the following genotypes were established for β-Lg gene: AA in 44.44 % cows and AB in 55.56 %, whilst BB genotype was not found. These results indicate that Busha cows had a higher presence of A allelic forms of both genes (k-CN and β-lactoglobulin) than HF cows.
Keywords
κ-casein; β-lactoglobulin; polymorphism; PCR-RFLP; Holstein-Friesian; Busha cattle
Hrčak ID:
161263
URI
Publication date:
6.7.2016.
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