Short communication, Note

Enhanced Production of Glucose Oxidase Using Penicillium notatum and Rice Polish

Shazia Sabir ; Industrial Biotechnology Laboratory, Department of Chemistry, University of Agriculture, 38040 Faisalabad, Pakistan
Haq Nawaz Bhatti ; Industrial Biotechnology Laboratory, Department of Chemistry, University of Agriculture, 38040 Faisalabad, Pakistan
Muhammad Anjum Zia ; Industrial Biotechnology Laboratory, Department of Chemistry, University of Agriculture, 38040 Faisalabad, Pakistan
Munir Ahmad Sheikh ; Industrial Biotechnology Laboratory, Department of Chemistry, University of Agriculture, 38040 Faisalabad, Pakistan

Abstract

Glucose oxidase (GOD) is an important enzyme that finds a wide range of applications in food and pharmaceutical industry. In this investigation the feasibility of using rice polish as a substrate for the production of GOD by Penicillium notatum in submerged fermentation (SmF) has been evaluated. The intention was to enhance total GOD activity by the selection of economical substrate, microorganism and consecutive optimization of various cultural conditions. Maximum GOD activity of (112±5) U/mL was achieved under optimum growth conditions: rice polish 5 g, incubation period 72 h, buffering agent 3 % (by mass per volume), incubation temperature (30±1) °C and pH=6.0. Addition of carbon and nitrogen sources further enhanced the enzyme yield, indicating an economically attractive process for GOD production.

Keywords

glucose oxidase; Penicillium notatum; submerged fermentation; rice polish; culture conditions

Hrčak ID:

24036

URI

https://hrcak.srce.hr/24036

Publication date:

13.12.2007.

Article data in other languages: croatian

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