Original scientific paper
Natural Antioxidant Constituents from Selected Aromatic Plants and Their Antimicrobial Activity Against Selected Pathogenic Microorganisms
Charalampos Proestos
; Laboratory of Food Chemistry, Agricultural University of Athens, Iera Odos 75, GR-118 55 Athens, Greece
Ioannis Spyridon Boziaris
; Laboratory of Food Chemistry, Agricultural University of Athens, Iera Odos 75, GR-118 55 Athens, Greece
Maria Kapsokefalou
; Laboratory of Food Chemistry, Agricultural University of Athens, Iera Odos 75, GR-118 55 Athens, Greece
Michael Komaitis
; Laboratory of Food Chemistry, Agricultural University of Athens, Iera Odos 75, GR-118 55 Athens, Greece
Abstract
Aromatic plants contain natural antioxidant constituents such as phenolic compounds, which have attracted a great deal of public and scientific interest because of their health-promoting effects as antioxidants. Five plants, Filipendula ulmaria (meadow sweet), Crataegus monogyna (hawthorn), Polygonum aviculare (polygonum), Potentilla anserina (silverweed),and Pelargonium purpureum (little robin), have been examined in order to determine their phenolic composition. Reversed-phase high performance liquid chromatography (RP-HPLC) was employed for the identification and quantification of phenolic compounds ofthe aforementioned plants. Gas chromatography–mass spectrometry method (GC-MS) was also used for identification of phenolic compounds after silylation. Analysis of the non-volatile and thermolabile phenolic compounds by GC-MS presupposes their conversion intovolatile and thermotolerant derivatives. The derivatization process was optimized againstreagents, temperature and reaction time. The antioxidant capacity was determined in driedplants and in their methanol extracts with the Rancimat test using sunflower oil as substrate. Both pulverized plants and extracts showed antioxidant capacity. Total phenoliccontent in the extracts was determined spectrometrically applying the Folin-Ciocalteu assay and it ranged from 7.2 to 28.2 gallic acid equivalents (GAE)/(mg/mL). Antimicrobial activity of the extracts against selected microorganisms was performed using the disk diffusion method. Gram-(+) bacteria were more sensitive to the plant extracts than Gram-(–) bacteria.
Keywords
plant antioxidants; RP-HPLC; GC-MS; trimethylsilyl derivatives; antimicrobial activity
Hrčak ID:
24399
URI
Publication date:
13.6.2008.
Visits: 4.674 *