Original scientific paper
https://doi.org/10.31895/hcptbn.16.1-2.6
A study on the bıoactıvıty of plant extracts obtaıned from Arum maculatum leaves by dıfferent extractıon technıques
Nazan Comlekcioglu
Sevilay Çolak
Ashabil Aygan
Abstract
Arum maculatum is a highly known plant worldwide for traditional use. The aim of this study is to evaluate the bioactivity of the plant extract
obtained using different techniques and solutions. Total fenolic, flavonoid components and antioxidant, antimicrobial and enzyme inhibition activity
of the plant extracts (Boiling in water, fermenting in water and USB in methanol) were investigated. Additionally, oil components of the extracts
was analysed in GC-MS. As a result of the GC-MS analysis, 18 different fatty acid were determined. Major fatty acid components of extracts were
palmitic acid (19.57%), oleic acid (15.25%), linoleic acid (21.84%) and alpha-linolenic acid (15.95%). The plant extracts were also found to be
consisting of omega 3-6-9 fatty acid. The results showed that methanolic extracts in USB is produced better and more effective findings than the
other extracts. According to the antimicrobial activity experiments, E. coli and S. aureus were the only strains inhibited by the all extracts obtained
three different methods. The highest inhibition was recorded against Bacillus subtilis with USB methanol extracts. The only antifungal activity
was observed against C. albicans with extracts obtained by boiling in water. Enzyme inhibition activity was very limited with all extracts. Amylase
activity was slightly inhibited (14.1%) up to 30 min.
Keywords
Arum maculatum; antioxidant activity; antimicrobial activity; enzyme inhibition; omega fatty acids
Hrčak ID:
265097
URI
Publication date:
1.6.2021.
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