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Food Technology and Biotechnology, Vol. 45 No. 1, 2007.

Original scientific paper

Preparation of a Whey-Based Probiotic Product with Lactobacillus reuteri and Bifidobacterium bifidum

Adrian Hernandez-Mendoza ; UNIDA-Instituto Tecnologico de Veracruz, M.A. de Quevedo #2779, Col. Formando Hogar, Veracruz, Veracruz 91897, México
Victor J. Robles ; UNIDA-Instituto Tecnologico de Veracruz, M.A. de Quevedo #2779, Col. Formando Hogar, Veracruz, Veracruz 91897, México
Jesus Ofelia Angulo ; UNIDA-Instituto Tecnologico de Veracruz, M.A. de Quevedo #2779, Col. Formando Hogar, Veracruz, Veracruz 91897, México
Javier De La Cruz ; UNIDA-Instituto Tecnologico de Veracruz, M.A. de Quevedo #2779, Col. Formando Hogar, Veracruz, Veracruz 91897, México
Hugo S. Garcia ; UNIDA-Instituto Tecnologico de Veracruz, M.A. de Quevedo #2779, Col. Formando Hogar, Veracruz, Veracruz 91897, México

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Abstract

Lactobacillus reuteri and Bifidobacterium bifidum were inoculated (2.8·10^8 and 4.7·10^8 CFU/mL, respectively) into reconstituted whey containing sucrose and pectin in order to prepare a fermented probiotic product. Inoculation levels were: 0.5, 1 or 2 % for Lactobacillus reuteri and 0.5 or 1 % for Bifidobacterium bifidum. The treatment with the highest bacterial counts and sensory scores was selected and stored at 4 °C for 30 days. Microbial counts, changes in pH values, titratable acidity and both triangle test and sensory attributes were monitored on the stored product. The beverage fermented for approx. 11 h and prepared with 2 % Lactobacillus reuteri and 0.5 % Bifidobacterium bifidum met the probiotic criterion by maintaining both bacterial populations at counts greater than 10^6 CFU/mL for the whole storage period. Titratable acidity and pH values as well as sensory properties did not change appreciably during the first 14 days of storage. At the end of the storage period (30 days), slight acidification was detected, although the beverage still retained an acceptable flavour.

Keywords

lactic acid bacteria; probiotics; Lactobacillus reuteri; Bifidobacterium bifidum; whey; fermented beverage

Hrčak ID:

30432

URI

https://hrcak.srce.hr/30432

Publication date:

15.3.2007.

Article data in other languages: croatian

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