Original scientific paper
Production of β-Fructofuranosidase by Arthrobacter sp. and Its Application in the Modification of Stevioside and Rebaudioside A
Zhong-Wei Xu
; College of Light Industry and Food Sciences, South China University of Technology, CN-510640, Guangzhou, PR China
Yu-Qiang Li
; College of Light Industry and Food Sciences, South China University of Technology, CN-510640, Guangzhou, PR China
Yong-Hua Wang
; College of Light Industry and Food Sciences, South China University of Technology, CN-510640, Guangzhou, PR China
Bo Yang
; School of Bioscience and Bioengineering, South China University of Technology, CN-510006, Guangzhou, PR China
Zheng-Xiang Ning
; College of Light Industry and Food Sciences, South China University of Technology, CN-510640, Guangzhou, PR China
Abstract
Arthrobacter sp. 10137 has been used to produce β-fructofuranosidase (FFase). Sucrose and corn steep powder in an optimized ratio of 10:1 were the best carbon and nitrogen sources for enzyme production in a shake flask. The maximum FFase activity was 26.69 U/mL after 22.5 h in batch culture, and the crude FFase, obtained by ultrafiltration and (NH4)2SO4 fractionation, was purified about 7-fold as measured by specific activity from the crude culture filtrate. The FFase was specific for introduction of a fructose molecule at the C19 position on both the stevioside and rebaudioside A, with high transfructosylating activity of 65 % after 15 h of incubation.
Keywords
β-fructofuranosidase; purification; stevioside and rebaudioside A; structure modification
Hrčak ID:
38102
URI
Publication date:
9.6.2009.
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