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Original scientific paper

Molecular identification of the brown algae, Cystoseira spp. (Phaeophycae, Fucales) from the Adriatic Sea – preliminary results

Srđana ROŽIĆ ; Institute of Oceanography and Fisheries, Laboratory for Benthos, Šetalište I. Meštrovića 63, 21000 Split, Croatia
Jasna PUIZINA orcid id orcid.org/0000-0001-7097-7261 ; University of Split, Faculty of Science, Department of Biology, Teslina 12, Croatia
Ivica ŠAMANIĆ ; University of Split, Faculty of Science, Department of Biology, Teslina 12, Croatia
Ante ŽULJEVIĆ ; Institute of Oceanography and Fisheries, Laboratory for Benthos, Šetalište I. Meštrovića 63, 21000 Split, Croatia
Boris ANTOLIĆ ; Institute of Oceanography and Fisheries, Laboratory for Benthos, Šetalište I. Meštrovića 63, 21000 Split, Croatia


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Abstract

In the attempt to identify an appropriate molecular marker which will enable genetic distinction between different Cystoseira species from the Adriatic Sea, two mitochondrial molecular markers were tested: the mt 23S rDNA and the mt23S-tRNAVal spacer. Two species were studied: Cystoseira spinosa and Cystoseira squarrosa. Sequence analyses showed no variation in the mt 23S rDNA among all individuals analyzed. But the analysis of the mt23S-tRNAVal spacer showed a differentiation between three haplotypes named A, B and C. The most abundant haplotype A was found in equal number in both species, while haplotype B was found only in C. spinosa and haplotype C was found only in C. squarrosa. However, when comparing to sequences available for several selected Mediterranean Cystoseira species, the mt23S-tRNAVal spacer failed to discriminate between species. Although these results indicate a limited use of the mitochondrial mt23S-tRNAVal intergenic spacer for discrimination among Adriatic Cystoseira species, they could also be interpreted as a sign of conspecificity of the investigated species or the reflection of a recent radiation. Further analysis will be necessary to improve molecular identification of these brown algae.

Keywords

Cystoseira; Adriatic Sea; mitochondrial DNA; molecular markers; molecular phylogeny

Hrčak ID:

97276

URI

https://hrcak.srce.hr/97276

Publication date:

30.12.2012.

Article data in other languages: croatian

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