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Molecular identification of the brown algae, Cystoseira spp. (Phaeophycae, Fucales) from the Adriatic Sea – preliminary results

Srđana ROŽIĆ ; Institut za oceanografiju i ribarstvo, Laboratorij za bentos, Šetalište I. Meštrovića 63, 21000 Split, Hrvatska
Jasna PUIZINA orcid id orcid.org/0000-0001-7097-7261 ; Sveučilište u Splitu, Prirodoslovno – matematički fakultet, Odjel za biologiju, Teslina 12, Hrvatska
Ivica ŠAMANIĆ ; Sveučilište u Splitu, Prirodoslovno – matematički fakultet, Odjel za biologiju, Teslina 12, Hrvatska
Ante ŽULJEVIĆ ; Institut za oceanografiju i ribarstvo, Laboratorij za bentos, Šetalište I. Meštrovića 63, 21000 Split, Hrvatska
Boris ANTOLIĆ ; Institut za oceanografiju i ribarstvo, Laboratorij za bentos, Šetalište I. Meštrovića 63, 21000 Split, Hrvatska


Puni tekst: engleski pdf 526 Kb

str. 447-455

preuzimanja: 893

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Sažetak

In the attempt to identify an appropriate molecular marker which will enable genetic distinction between different Cystoseira species from the Adriatic Sea, two mitochondrial molecular markers were tested: the mt 23S rDNA and the mt23S-tRNAVal spacer. Two species were studied: Cystoseira spinosa and Cystoseira squarrosa. Sequence analyses showed no variation in the mt 23S rDNA among all individuals analyzed. But the analysis of the mt23S-tRNAVal spacer showed a differentiation between three haplotypes named A, B and C. The most abundant haplotype A was found in equal number in both species, while haplotype B was found only in C. spinosa and haplotype C was found only in C. squarrosa. However, when comparing to sequences available for several selected Mediterranean Cystoseira species, the mt23S-tRNAVal spacer failed to discriminate between species. Although these results indicate a limited use of the mitochondrial mt23S-tRNAVal intergenic spacer for discrimination among Adriatic Cystoseira species, they could also be interpreted as a sign of conspecificity of the investigated species or the reflection of a recent radiation. Further analysis will be necessary to improve molecular identification of these brown algae.

Ključne riječi

Cystoseira, Adriatic Sea, mitochondrial DNA, molecular markers, molecular phylogeny

Hrčak ID:

97276

URI

https://hrcak.srce.hr/97276

Podaci na drugim jezicima: hrvatski

Posjeta: 1.598 *