Original scientific paper

Temperature-dose relationships with aflatoxin M1 in milk on the brine shrimp (Artemia salina) larvae

Lejla Duraković ; Faculty of Food Technology and Biotechnology, Laboratory for General and Food Microbiology, University of Zagreb, Pierottijeva 6, Zagreb, Croatia
Mihaela Blažinkov
Andrea Skelin
Sanja Sikora
Frane Delaš
Mirna Mrkonjić-Fuka
Katarina Huić-Babić
Sulejman Redžepović

Abstract

Temperature-dose relationships with aflatoxin M1 (AFM1) were studied using the brine shrimp Artemia salina larvae as an biological indicator in the temperature range from 20 °C to 40 °C. Increase in the incubation temperature resulted in sensitivity increase by the brine shrimp to AFM1. Optimum sensitivity occured at 30 °C. Positive results were obtained at 0.18 μg AFM1 x L-1 of whole pasteurized milk with a mortality of over 15%. Greater than 90 % mortality occurred at dose levels of 0.9 μg AFM1 x L-1 and above. The test can be conducted during 30-60 hours.

Keywords

aflatoxin M1; bioassay; Artemia salina; LC50 and T50 doses

Hrčak ID:

69095

URI

https://hrcak.srce.hr/69095

Publication date:

14.6.2011.

Article data in other languages: croatian

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