Original scientific paper
A Comparative Analysis of Recombinant Expression and Solubility Screening of Two Phytases in E. coli
Ushasree Mrudula Vasudevan
; Biotechnology Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, 695 019 Trivandrum, Kerala, India
Sumayya Husaiba Beevi Salim
; Biotechnology Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, 695 019 Trivandrum, Kerala, India
Ashok Pandey
orcid.org/0000-0003-1626-3529
; Biotechnology Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, 695 019 Trivandrum, Kerala, India
Abstract
Microbial phytases, especially from fungal and bacterial sources, have received much attention as food additives in human nutrition and as feed supplements for monogastric animals. An effective expression screening method for recombinant production of this enzyme on a small scale is industrially desirable. An effort has been made in this work to clone and express phytase genes from Aspergillus sp. and Escherichia coli with the selected host, vector and inducer combination. Albeit the formation of insoluble inclusion bodies by fungal phytase, recombinant E. coli appA was effectively expressed in a cost-effective manner in the periplasm of BL21plysS using an inducer concentration of 0.01 mM in 4 h of growth. Enzyme was purified in three consecutive steps and functional studies were carried out.
Keywords
A. niger phytase; inducer concentration; recombinant appA
Hrčak ID:
71056
URI
Publication date:
10.8.2011.
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