Short communication, Note
Ractopamine and Clenbuterol Urinary Residues in Pigs as Food-Producing Animals
Jelka Pleadin
orcid.org/0000-0002-0768-0462
; Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Savska 143, HR-10000 Zagreb, Croatia
Ana Vulić
; Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Savska 143, HR-10000 Zagreb, Croatia
Nina Perši
; Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Savska 143, HR-10000 Zagreb, Croatia
Dinka Milić
; Dubravica Swine Farm, Ltd., Pavla Štoosa 109, HR-10293 Dubravica, Croatia
Nada Vahčić
; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia
Abstract
The aim of the study is to determine residual ractopamine (RCT) and clenbuterol (CLB) concentrations in urine during and after their administration in anabolic dose to male pigs. RCT and CLB residues were determined using previously validated enzyme-linked immunosorbent assay (ELISA) as a quantitative screening method. Hydrolysis of urine samples with β-glucuronidase showed significantly higher (p<0.05) RCT residues. Study results showed RCT and CLB urine concentrations to vary greatly during oral treatment for 28 days, with maximal RCT and CLB concentration recorded on day 25 ((327.4±161.0) ng/mL) and day 20 ((68.4±32.2) ng/mL), respectively. RCT concentration of (57.1±10.6) ng/mL and CLB concentration of (38.8±20.1) ng/mL were measured on day 0 of treatment withdrawal; on day 7 of treatment withdrawal, the measured concentration of RCT ((5.0±0.9) ng/mL) was 20-fold of CLB concentration ((0.3±0.2) ng/mL). Study results indicate that the excretion of RCT and CLB in pig urine could clearly point to their abuse in pigs as food-producing animals, in particular when using sample hydrolysis with β-glucuronidase on RCT determination.
Keywords
ractopamine; clenbuterol; ELISA; pig; urine
Hrčak ID:
75192
URI
Publication date:
20.12.2011.
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