Agriculture, Vol. 18 No. 2, 2012.
Original scientific paper
COMPARATIVE ELECTROPHORETIC PATTERNS OF ALBUMINS/ GLOBULINS EXTRACTED FROM DRY GRAINS AND GREEN MALTS OF BARLEY VARIETIES
Ivica Strelec
orcid.org/0000-0003-3999-8499
; J.J. Strossmayer University of Osijek, Faculty of Food Technology Osijek, Croatia
Elizabeta Has-Schön
; J.J. Strossmayer University of Osijek, Department of Biology, Osijek, Croatia
Ljubinka Vitale
; Rudjer Bošković Institute, Zagreb, Croatia
Abstract
Applicability of electrophoretic separations of albumins/globulins, followed by nonspecific
protein staining and specific glycoprotein and aminopeptidase detection has been examined for barley variety discrimination. Albumins/globulins extracted from dry grains and green malt of six barley varieties were separated by polyacrylamide gel electrophoresis (10 % T, pH=8.9) of native and sodium dodecyl sulfate denatured proteins, and by isoelectric focusing in pH gradient of 3.5-9.5 and 4.0-6.5. Analysis of dry grain extracts gave better results than green malt extracts. Obtained data indicate that SDS-PAGE of albumins/globulins and IEF in pH gradient 3.5-9.5 followed by Coomassie Blue staining could be useful in Croatian barley variety discrimination. Angora barley could be clearly distinguished from the other varieties which were grouped as follows: Rodnik/Sladoran, Barun/Rex, and Martin. Glycoprotein patterns did not improve the recognition of individual varieties. Broad specificity, phenylalanine and leucine preferring, and arginine specific aminopeptidase were not found as applicable markers for discrimination of examined barley varieties.
Keywords
barley variety discrimination; electrophoretic separations; glycoprotein detection; aminopeptidase activity detection
Hrčak ID:
94628
URI
Publication date:
17.12.2012.
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