Original scientific paper
Production of single-cell proteins by yeast Kluyveromyces fragilis on whey
Slobodan Grba
; Prehrambeno-biotehnološki fakultet, Pierottijeva 6, 10000 Zagreb
Vesna Stehlik-Tomas
Abstract
The possibility of cultivation of the yeast Kluyveromyces fragilis for the production of single-cell protein on deproteinated whey was studied. The hatch and the continuous cultivations were performed at pH 5.0-5.5 and the temperature of 40 °C. By experiments on the shaker and in laboratory fermenter it was established that the yeast cells grow well on 2 % deproteinated whey with the addition of ammonium sulphate and potassium hydrogen phosphate. At optimal conditions the biomass yield was 0.45-0.55 g DM/g lactose with the specific growth rate up to 0.25 h^-1 and productivity of 1.3 g/L/h. When 0.1 % of the yeast extract was added to the basic medium the yeast cells were multiplying faster (μ = 0.35-0.38 h^-1) and productivity was up to 1.8 g/L/h. If the water necessary for the dilution of whey was exchanged with molasses slop a. very good effect attained in the cultivation of yeast cells. The specific growth rate was 0.4 h^-1 and productivity up to 2.4 g/L/h.
Keywords
whey; single-cell proteins; Kluyveromyces fragilis
Hrčak ID:
96077
URI
Publication date:
4.5.1985.
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