Original scientific paper

Optimization of Amylase and Protease Production from Aspergillus awamori in Single Bioreactor Through EVOP Factorial Design Technique

Sangeeta Negi ; Agriculture and Food Engineering Department, Microbial Biotechnology and Downstream Processing Laboratory, IIT Kharagpur, West Bengal 721302, India
Rintu Banerjee ; Agriculture and Food Engineering Department, Microbial Biotechnology and Downstream Processing Laboratory, IIT Kharagpur, West Bengal 721302, India

Abstract

Evolutionary operation (EVOP) factorial design technique was explored in order to economically produce amylase and protease at their optimum level in a single bioreactor by modified solid-state fermentation. Maximum yields of amylase and protease were achieved, using wheat bran as a substrate by a highly potent, locally isolated strain of Aspergillus awamori: Nakazawa MTCC 6652. The strain had been induced previously, inferring the ability to produce both enzymes concomitantly in a single bioreactor with their maximum capacity. The highest secretion of amylase and protease were measured to be 9420.6 and 1930 U/g, respectively, at 37 °C. pH and relative humidity were found to be optimum at 4 and 85 %, evaluated through EVOP method.

Keywords

amylase; protease; SSF; Aspergillus awamori; EVOP; production

Hrčak ID:

109888

URI

https://hrcak.srce.hr/109888

Publication date:

15.6.2006.

Article data in other languages: croatian

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