Original scientific paper

Solid Culturing of Bacillus amyloliquefaciens for α-Amylase Production

Dhanya Gangadharan ; Biotechnology Division, Regional Research Laboratory, CSIR, Trivandrum 695019, India
Swetha Sivaramakrishnan ; Biotechnology Division, Regional Research Laboratory, CSIR, Trivandrum 695019, India
Kesavan Madhavan Nampoothiri ; Biotechnology Division, Regional Research Laboratory, CSIR, Trivandrum 695019, India
Ashok Pandey orcid.org/0000-0003-1626-3529 ; Biotechnology Division, Regional Research Laboratory, CSIR, Trivandrum 695019, India

Abstract

Fourteen different agroresidues were screened for alpha amylase production using Bacillus amyloliquefaciens ATCC 23842. Among them, wheat bran (WB) and groundnut oil cake (GOC) in mass ratio of 1:1 was proved as the best substrate source. Supplementation with 0.01 M KH2PO4 and 1 % soluble starch enhanced the enzyme yield considerably. Maximum enzyme recovery from the solid mass was obtained when extracted with 0.1 M acetate buffer, pH=5.0. Maximum enzyme titer expressed as units per mass of dry substrate obtained was 62 470 U/g after 72 hours of fermentation at 37 °C by using the above solid substrate mixture (5 g) with the initial moisture of 85 % and inoculated with Bacillus amyloliquefaciens of 2·109 CFU/mL.

Keywords

α-amylase; Bacillus amyloliquefaciens; solid-state fermentation; agroresidues

Hrčak ID:

109896

URI

https://hrcak.srce.hr/109896

Publication date:

15.6.2006.

Article data in other languages: croatian

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