Preliminary communication

Effects of Various Process Parameters on the Production of γ-Linolenic Acid in Submerged Fermentation

Syed U. Ahmed ; Biotechnology Division, Regional Research Laboratory (Council of Scientific and Industrial Research), Industrial Estate PO, Pappanamcode, Trivandrum 695019, India
Sudheer K. Singh ; Biotechnology Division, Regional Research Laboratory (Council of Scientific and Industrial Research), Industrial Estate PO, Pappanamcode, Trivandrum 695019, India
Ashok Pandey ; Biotechnology Division, Regional Research Laboratory (Council of Scientific and Industrial Research), Industrial Estate PO, Pappanamcode, Trivandrum 695019, India
Sanjit Kanjilal ; Oils and Fats Division, Indian Institute of Chemical Technology, Hyderabad 500007, India
Rachapudi B. N. Prasad ; Oils and Fats Division, Indian Institute of Chemical Technology, Hyderabad 500007, India

Abstract

Studies were carried out on the fermentative production of γ-linolenic acid (GLA) using seven strains belonging to Mucorales. An oleaginous fungal strain, isolated from the Western Ghats of Kerala produced GLA at a level of 8 % (by mass), when grown in a complex medium containing glucose as the sole carbon source. Effects of different culture conditions were investigated in shake flasks. Maximum dry biomass and total GLA obtained were 48.4 g/L and 636 mg/L, respectively, in the culture cultivated at 30 °C and 200 rpm for 7 days. Among the organic nitrogen sources investigated, yeast extract, and combination of corn steep liquor and baker’s yeast in 1:1 ratio were useful for enhancing the GLA production and the effects were comparable.

Keywords

γ-linolenic acid; submerged fermentation; Mucor sp.; process parameters

Hrčak ID:

109905

URI

https://hrcak.srce.hr/109905

Publication date:

15.6.2006.

Article data in other languages: croatian

Visits: 2.031 *