Original scientific paper

Intrinsic Activity of Inulinase from Kluyveromyces marxianus ATCC 16045 and Carbon and Nitrogen Balances

Bernardo Onagar Yépez Silva-Santisteban ; Department of Food Engineering, FEA-University of Campinas, Campinas, SP, CEP 13083-970, CP 6121, Brazil
Attilio Covenrti ; Department of Chemical and Process Engineering »G.B. Bonino«, University of Genoa, Via Opera Pia 15, I–16145 Genoa, Italy
Francisco Maugeri Filhob ; Department of Food Engineering, FEA-University of Campinas, Campinas, SP, CEP 13083-970, CP 6121, Brazil

Abstract

Kluyveromyces marxianus ATCC 16045 was cultivated in a batch on minimal medium to overproduce inulinase. The fermented broth was purified by fast protein liquid chromatography and ultrafiltration, and a pure enzyme fraction was obtained. SDS-PAGE electrophoresis allowed calculating molecular mass of 59 kDa, while nitrogen determination by the micro-Kjeldahl method allowed evaluating intrinsic inulinase activity of 879 IU/mg. These results were then used to perform material balances of the fermentation process, which suggested that no more than 0.1 % either of carbon or nitrogen initially present in the medium were incorporated in the extracellular inulinase released under different cultivation conditions. The information obtained in this study can be used for future proposal of metabolic models describing inulinase overproduction by this yeast.

Keywords

carbon source; nitrogen source; inulinase production; Kluyveromyces marxianus

Hrčak ID:

110084

URI

https://hrcak.srce.hr/110084

Publication date:

15.12.2006.

Article data in other languages: croatian

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