Short communication, Note

Validation of a Quick PCR Method Suitable for Direct Sequencing: Identification of Fusarium Fungal Species and Chemotypes for Preventive Approaches in Food Safety

Marine Pallez ; Public Research Centre Gabriel Lippmann, Environment and Agro-Biotechnologies Department, 41, rue du Brill, LU-4422 Belvaux, Luxembourg
Matias Pasquali ; Public Research Centre Gabriel Lippmann, Environment and Agro-Biotechnologies Department, 41, rue du Brill, LU-4422 Belvaux, Luxembourg
Torsten Bohn ; Public Research Centre Gabriel Lippmann, Environment and Agro-Biotechnologies Department, 41, rue du Brill, LU-4422 Belvaux, Luxembourg
Lucien Hoffmann ; Public Research Centre Gabriel Lippmann, Environment and Agro-Biotechnologies Department, 41, rue du Brill, LU-4422 Belvaux, Luxembourg
Marco Beyer ; Public Research Centre Gabriel Lippmann, Environment and Agro-Biotechnologies Department, 41, rue du Brill, LU-4422 Belvaux, Luxembourg

Abstract

Species determination by sequencing and PCR genetic chemotyping, used to determine the toxigenic potential of Fusarium strains, is fundamental for developing preventive strategies in food safety. Here we propose and statistically validate a quick protocol for standardizing
the procedure of species determination by sequencing of the elongation factor 1-α and multiplex genetic chemotyping using the Tri12 gene, based on fungal growth on Miracloth tissue coupled with microwave extraction. The test was validated on 75 Fusarium culmorum and Fusarium graminearum strains.

Keywords

Fusarium culmorum; Fusarium graminearum; microwave DNA extraction, EF1-α

Hrčak ID:

126185

URI

https://hrcak.srce.hr/126185

Publication date:

15.9.2014.

Article data in other languages: croatian

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