Short communication, Note
Optimization, Purification and Characterization of Polygalacturonase from Mango Peel Waste Produced by Aspergillus foetidus
Sudheer Kumar Yannam
; Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke West Montreal-H4B2E6, Canada
Prakasham Reddi Shetty
; Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad-500007, India
Vijaya Sarathi Reddy Obulum
; Department of Biochemistry, Sri Venkateswara University, Tirupati-517502, India
Abstract
Pectin-rich mango peel from industrial waste was used as a substrate for the production and characterization of novel polygalactauronase enzyme. Its production was optimized with five important factors; KH2PO4, pH, peptone, MgSO4 and urea by employing response
surface methodology using submerged fermentation with Aspergillus foetidus. Total of 50 experimental runs were carried out and the predicted values for optimization were in good agreement with experimental data. The results showed that a satisfactory production
of polygalacturonase from the mango peel could be achieved, reaching up to 36.5 U/mL under optimized medium conditions of pH=5.8 and (in %, by mass per volume): KH2PO4 0.22, peptone 0.5, MgSO4 0.02 and urea 0.2 %. The polygalacturonase was partially
purified to 3.4-fold and the molecular mass was found to be 34 kDa. The optimum pH and temperature for polygalacturonase activity were 5 and 55 °C, respectively.
Keywords
mango peel; Aspergillus foetidus; submerged fermentation; polygalacturonase; response surface methodology
Hrčak ID:
126186
URI
Publication date:
15.9.2014.
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