Original scientific paper
https://doi.org/10.17113/ftb.52.04.14.3701
Identification of Differentially Expressed Genes by cDNA-AFLP Technique in Response to Drought Stress in Triticum durum
Marouane Melloul
; Genetic and Biometry Laboratory, Faculty of Sciences, University Ibn Tofail, BP 133, 14000 Kenitra, Morocco
Driss Iraqi
; National Institute of Agronomical Research, Avenue de la Victoire, BP 415, Rabat, Morocco
MyAbdelaziz El Alaoui
; Genetic and Biometry Laboratory, Faculty of Sciences, University Ibn Tofail, BP 133, 14000 Kenitra, Morocco
Gilles Erba
; Labgene Scientifi c Instruments, Athens Building, Business Park, 74160 Archamps, France
Sanaa Alaoui
; Functional Genomic Platform, Technical Unit (UATRS), National Center for Scientifi c and Technical Research (CNRST), Angle Allal Fassi, Avenue des FAR, Hay Riad, BP 8027, 10102 Rabat, Morocco
Mohammed Ibriz
; Genetic and Biometry Laboratory, Faculty of Sciences, University Ibn Tofail, BP 133, 14000 Kenitra, Morocco
Elmostafa Elfahime
; Functional Genomic Platform, Technical Unit (UATRS), National Center for Scientifi c and Technical Research (CNRST), Angle Allal Fassi, Avenue des FAR, Hay Riad, BP 8027, 10102 Rabat, Morocco
Abstract
Drought is the single largest abiotic stress factor leading to reduced crop yields. The identification of diff erentially expressed genes and the understanding of their functions in environmentally stressful conditions are essential to improve drought tolerance. Transcriptomics is a powerful approach for the global analysis of molecular mechanisms under abiotic stress. To identify genes that are important for drought tolerance, we analyzed mRNA populations from untreated and drought-stressed leaves of Triticum durum by cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique. Overall, 76 transcript-derived fragments corresponding to differentially induced transcripts were successfully sequenced. Most of the transcripts identified here, using basic local alignment search tool (BLAST) database, were genes belonging to different functional categories related to metabolism, energy, cellular biosynthesis, cell defense, signal transduction, transcription regulation, protein degradation and transport. The expression patterns of these genes were confirmed by quantitative reverse transcriptase real-time polymerase chain reaction (qRT-PCR) based on ten selected genes representing different patterns. These results could facilitate the understanding of cellular mechanisms involving groups of genes that act in coordination in response to stimuli of water deficit. The identification of novel stress-responsive genes will provide useful data that could help develop breeding strategies aimed at improving durum wheat tolerance to field stress.
Keywords
cDNA-AFLP; drought stress; transcript-derived fragments; durum wheat; real-time PCR
Hrčak ID:
130941
URI
Publication date:
15.12.2014.
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