Original scientific paper

HPLC DEIERMINATION OF CATECEINS IN GRAPE SEEDS OF PLAVAC MALI CULTIVAR

Višnja Katalinić ; Institut za jadranske kulture i melioraciju krša, Split, Hrvatska
Petar Maleš ; Institut za jadranske kulture i melioraciju krša, Split, Hrvatska
Ljubica Šimac ; Institut za jadranske kulture i melioraciju krša, Split, Hrvatska

Abstract

Using HPLC technique on reversed-phase, the amounts of (+) - catechin and (-) epicatechin in grape seeds of autochthonous Dalmatian cultivar plavac mali, were analyzed during the growth season. To prevent interference between phenolic compounds, because many of them have similar UV absorption spectra, we fractionated acidic and neutral phenolic compounds on Sep Pak C 18 cartridges. Catechins were separated using HPLC with gradient solvent system at 280 mm. The grape seeds were analyzed during various stages of ripening. The results show the highest concentrations of catechins in the verison. The level decrease quickl troughout maturation. The major flavan- 3-ol monomer was (+) - catechin.

Keywords

calauring stage; grape seeds; technological ripening stage

Hrčak ID:

142605

URI

https://hrcak.srce.hr/142605

Publication date:

21.3.1998.

Article data in other languages: croatian

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