Original scientific paper

https://doi.org/10.17113/ftb.53.04.15.4176

Sensitive Voltammetric Determination of Natural Flavonoid Quercetin on a Disposable Graphite Lead

Dai Long Vu ; University of Pardubice, Studentska 573, CZ-53210 Pardubice, Czech Republic
Simona Žabčíková ; University of Pardubice, Studentska 573, CZ-53210 Pardubice, Czech Republic
Libor Červenka ; University of Pardubice, Studentska 573, CZ-53210 Pardubice, Czech Republic
Bensu Ertek ; Canakkale Onsekiz Mart University, Mart University 18, TR-17100 Canakkale, Turkey
Yusuf Dilgin ; Canakkale Onsekiz Mart University, Mart University 18, TR-17100 Canakkale, Turkey

Abstract

In this paper, a pencil graphite electrode was pretreated using chronoamperometry technique in phosphate buffer solution (pH=7.0) for sensitive determination of quercetin. Oxidation of quercetin was investigated using pretreated pencil graphite electrode and anodic stripping differential pulse voltammetry. Under optimal conditions, the anodic current of quercetin exhibited linear response to its concentration in the range from 0.001 to 1.5 μmol/L with the limit of detection of 0.3·10–3 μmol/L. The proposed method was successfully applied for the determination of quercetin in cranberry and blackcurrant juices with recovery rate from 93.2 to 94.7 %. Solid-phase extraction was found to be necessary prior to voltammetric determination of quercetin in fruit juice samples using pretreated pencil graphite electrode.

Keywords

pencil graphite electrode; voltammetry; quercetin; fruit juices

Hrčak ID:

150128

URI

https://hrcak.srce.hr/150128

Publication date:

28.12.2015.

Article data in other languages: croatian

Visits: 1.831 *