Original scientific paper

https://doi.org/10.5599/admet.4.1.277

Reversed phase parallel artificial membrane permeation assay for log P measurement

Zihao Song ; Department of pharmaceutics, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1, Miyama, Funabashi, Chiba, 274-8510, Japan
Katsuhide Terada ; Department of pharmaceutics, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1, Miyama, Funabashi, Chiba, 274-8510, Japan
Kiyohiko Sugano ; Department of pharmaceutics, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1, Miyama, Funabashi, Chiba, 274-8510, Japan

Abstract

A reversed phase parallel artificial membrane permeation assay (RP-PAMPA) was newly invented for log P measurement. An oil/water/oil sandwich was constructed using a conventional PAMPA instrument. 1 % agarose was used to improve the physical stability of the water phase. A linear correlation between log P and the apparent permeability was observed in the -0.24 < log P < 2.85 region (R2 = 0.98). RP-PAMPA was also applied to pKa measurement.

Keywords

octanol; partition; parallel artificial membrane permeation assay; pKa; drug

Hrčak ID:

154845

URI

https://hrcak.srce.hr/154845

Publication date:

31.3.2016.

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