ADMET and DMPK, Vol. 4 No. 1, 2016.
Izvorni znanstveni članak
https://doi.org/10.5599/admet.4.1.277
Reversed phase parallel artificial membrane permeation assay for log P measurement
Zihao Song
; Department of pharmaceutics, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1, Miyama, Funabashi, Chiba, 274-8510, Japan
Katsuhide Terada
; Department of pharmaceutics, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1, Miyama, Funabashi, Chiba, 274-8510, Japan
Kiyohiko Sugano
; Department of pharmaceutics, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1, Miyama, Funabashi, Chiba, 274-8510, Japan
Sažetak
A reversed phase parallel artificial membrane permeation assay (RP-PAMPA) was newly invented for log P measurement. An oil/water/oil sandwich was constructed using a conventional PAMPA instrument. 1 % agarose was used to improve the physical stability of the water phase. A linear correlation between log P and the apparent permeability was observed in the -0.24 < log P < 2.85 region (R2 = 0.98). RP-PAMPA was also applied to pKa measurement.
Ključne riječi
octanol; partition; parallel artificial membrane permeation assay; pKa; drug
Hrčak ID:
154845
URI
Datum izdavanja:
31.3.2016.
Posjeta: 1.363 *