Short communication, Note
https://doi.org/10.17113/ftb.54.02.16.4095
Near-Infrared Spectroscopic Analysis of Total Phenolic Content and Antioxidant Activity of Berry Fruits
Jasenka Gajdoš Kljusurić
orcid.org/0000-0001-6657-7337
; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Kiril Mihalev
; University of Food Technologies, Department of Food Preservation and Refrigeration Technology, Plovdiv, Bulgaria
Ivana Bečić
; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Ivana Polović
; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Mariya Georgieva
; University of Food Technologies, Department of Food Preservation and Refrigeration Technology, Plovdiv, Bulgaria
Senka Djaković
orcid.org/0000-0002-6322-1770
; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Želimir Kurtanjek
orcid.org/0000-0001-5453-6255
; Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Abstract
This study evaluates the feasibility of using near-infrared (NIR) spectroscopy as a rapid and environmentally friendly technique for validation and prediction of the total phenolic content (TPC) and antioxidant activity (AOA) indices (as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, inhibition time (IT) of the Briggs-Rauscher oscillating reaction, and relative antioxidant capacity (RAC)) of berry fruit extracts. The analysed berry samples originated from Croatia (blackberries, wild blueberries, raspberries, red currants and strawberries) and Bulgaria (wild blueberries, raspberries and strawberries). Principal component analysis and partial least squares (PLS) regression were used from the set of chemometric tools in distinguishing and validating the measured berry fruit extract. ANOVA and PCA showed no significant impact of the origin and freshness of the samples. PLS models were developed to validate the relationship of NIR spectra with TPC and AOA of berry fruits. Representativeness of the models was expressed with the R2 and the ratio of performance to deviation. Calculated R2 values were above 0.84 and the ratio of performance to deviation was between 1.8 and 3.1, indicating adequacy of the PLS models.
Keywords
berry fruits; antioxidant activity; NIR spectroscopy; PLS models; total phenolic content
Hrčak ID:
160378
URI
Publication date:
24.6.2016.
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