Original scientific paper

Effects of abscisic acid and carbohydrates on the maturation of Picea omorika (Panč.) Purk. somatic embryos

Tamara Tramišak-Milaković ; Ruđer Bošković Institute, Department of Molecular Genetics, Zagreb, Croatia
Snježana Mihaljević ; Ruđer Bošković Institute, Department of Molecular Genetics, Zagreb, Croatia
Sibila Jelaska ; University of Zagreb, Faculty of Science, Department of Molecular Biology, Zagreb, Croatia

Abstract

In eight embryogenie tissue lines of omorika spruce the roles of carbohydrates and abscisic acid on embryo maturation were examined. Sucrose, glucose and maltose (3% and 6%) with abscisic acid (20, 40, 60 μM) were screened for optimal combination with respect to embryo maturation. The B lines, which expressed a brown tissue phenotype, achieved superior embryo maturation on 3% glucose and 40 μM abscisic acid. The VV lines with a white and translucent tissue phenotype showed the best maturation capacity on 3% sucrose or 3% maltose with 20 or 40 μM ABA. Sixty μM ABA inhibited embryo maturation. Media containing 6 % of either sucrose, glucose and maltose produced fewer mature embryos compared to media with 3% of the same sugars. The VV lines had almost three times lower maturation capacity in relation to the B lines.

Keywords

Picea omorika; omorika spruce; somatic embryogenesis; abscisic acid; embryo maturation; carbohydrates

Hrčak ID:

160606

URI

https://hrcak.srce.hr/160606

Publication date:

31.12.1999.

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