Original scientific paper

CONTROL OF GENETIC IDENTITY BY PROTEIN ELECTROPHORESIS IN VARIETAL REPRODUCTION OF WHEAT

Nenad Đurić ; Fakultet za biofarming, Bačka Topola, Srbija
Gorica Cvijanović ; Fakultet za biofarming, Bačka Topola, Srbija
Mirela Matković ; Fakultet za biofarming, Bačka Topola, Srbija
Gordana Dozet ; Fakultet za biofarming, Bačka Topola, Srbija
Gordana Branković ; Poljoprivredni fakultet, Beograd, Srbija

Abstract

Maintenance and control of genetic purity and genetic identity is one of the ultimate goals in seed production after the recognition of some varieties of wheat. The application of positive and negative selection at the phenotypic level in varietal reproduction of wheat, enabled 99 and 100% of varietal purity in crops of all seed categories. In order to study the genetic identity, in this paper the reserve proteins of wheat – gliadins were analyzed. They were separated by polyacrylic gel electrophoresis into numerous components that showed great heterogeneity. Of all the analyzed wheat varieties, only PKB Rodika variety had the genetic purity of 100% in all categories of seed. PKB Kristina had the genetic purity of 99% in the pre-basic seed category and the variety BG Merkur had genetic purity of 99% in the seed of first generation – C1. Protein electrophoresis showed there was the divergence among the varieties of wheat in its composition of gliadin fractions, while there was uniformity among defferent varietal reproductions within each variety.

Keywords

wheat; genetic identity; protein electrophoresis

Hrčak ID:

161765

URI

https://hrcak.srce.hr/161765

Publication date:

5.7.2016.

Article data in other languages: croatian

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