Original scientific paper
Amperometric Biosensor for Monitoring Respiration Activity of Saccharomyces cerevisiae in the Presence of Cobalt and Zinc
Željka Filipović-Kovačević
; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb Croatia
Miroslav Mikšaj
; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb Croatia
Nikola Berčuk
; Podravka d.d., Koprivnica, Croatia
Marijana Jukić
; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb Croatia
Abstract
For efficient control of heavy metal concentrations electrochemical methods, such as polarography and related techniques, are applied. Their advantages are simplicity, short analysis time and small quantities of samples needed. The presence of some heavy metals, such as zinc and cobalt, accelerates the growth of yeast. For the measurements of concentration changes, amperometric biosensor containing yeast Saccharomyces cerevisiae was used. The influence of zinc and cobalt on respiratory activity of the yeast Saccharomyces cerevisiae was estimated by measuring oxygen in the solution that was earlier enriched with cobalt or zinc. Measurements were performed using modified Clark’s oxygen electrode and the investigated concentrations of cobalt and zinc were up to 100 mg/L.
Keywords
biosensor; Saccharomyces cerevisiae; cobalt; zinc
Hrčak ID:
178435
URI
Publication date:
18.6.2002.
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