Short communication, Note
Lectins Labelled with Digoxin as a Novel Tool to Study Glycoconjugates
Gordan Lauc
; Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kova~i}a 1, HR-10000 Zagreb, Croatia
Jerka Dumić
; Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kova~i}a 1, HR-10000 Zagreb, Croatia
Sandra Šupraha
; Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kova~i}a 1, HR-10000 Zagreb, Croatia
Mirna Flögel
; Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kova~i}a 1, HR-10000 Zagreb, Croatia
Abstract
In recent years it has become clear that carbohydrate portions of glycoconjugates are performing numerous vital physiological functions in higher organisms. However, since glycobiology is a relatively new science, and carbohydrate structures are highly complex, the continuous development of novel analytical techniques is necessary to support the process of understanding the intricate nature of glycoconjugate structure and function. The introduction of digoxin as a novel tag for labelling of lectins that are being used to analyse glycoconjugates in immunoassay-like techniques is described. Lectins labelled with digoxin have significant advantages over biotin- or digoxigenin-labelled lectins and will hopefully prove to be a useful addition to the repertoire of glycobiological tools.
Keywords
lectins; glycoconjugates; digoxin; immunoassay-like techniques
Hrčak ID:
178540
URI
Publication date:
12.12.2002.
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