Original scientific paper

SOLVENT EXTRACTION AND CHROMATOGRAPHIC DETERMINATION OF POLYPHENOLS IN OLIVE POMACE

Dubravka Vitali Čepo ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, HR-10000 Zagreb, Croatia
Petra Albahari ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, HR-10000 Zagreb, Croatia
Marijana Zovko Končić ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, HR-10000 Zagreb, Croatia
Kristina Radić ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, HR-10000 Zagreb, Croatia
Sanja Jurmanović ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, HR-10000 Zagreb, Croatia
Mario Jug ; University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kovačića 1, HR-10000 Zagreb, Croatia

Abstract

Olive oil mill waste obtained after two-phase olive oil extraction process was subjected to conventional liquid solvent extraction under different pH/temperature/duration conditions using different types of food-grade solvents. The independent variables were: solvent type (ethanol percentage), extraction temperature (20-90 °C), extraction time (30 min-24 h) and pH (2-10.3) of extraction solvent while the response variables were total phenolic content and antioxidant activity of obtained extracts. The optimum solvent extraction conditions for phenols were 120 min at 70 °C using 60% ethanol as extraction solvent, at solvent to sample ratio 5:1 (v/w). For quantification of major bioactive olive polyphenols hydroxytyrosol, tyrosol and oleuropein in obtained extracts, fast and simple RP-HPLC-DAD method was developed and validated. Oleuropein was presented in highest amounts with average value of 115.14±0.19 mg/kg of fresh olive pomace.

Keywords

olive pomace; extraction; HPLC-DAD; hydroxytyrosol; tyrosol; oleuropein

Hrčak ID:

182916

URI

https://hrcak.srce.hr/182916

Publication date:

12.6.2017.

Visits: 3.295 *