Original scientific paper
Ex Vitro Rooting and Survival of Regenerated Shoots from three Fig (Ficus carica L.) Genotypes
Akhtar Shekafandeh
; Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz Iran
Seyed Tahereh Shahcheraghi
; Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz Iran
Abstract
The aim of this study was to optimize the rooting of regenerated shoots from nodal segments of three genotypes of fig named: ‘Bargchenari’, ‘Dehdez’ and ‘Runu’. For that purpose, the bottom of shoots (about 5 mm) was dipped in Indole-3-butyric acid (IBA) solution of different concentrations: 500, 1000, 1500, 2000 mg L-1 and control for 5 seconds and then cultured in sand medium. The test was performed in a completely randomized design with at least 5 replications. In ‘Runu’, the highest rooting percentage and the number of roots per shoot (100% and 15.67, respectively) were observed in concentration of 2000 mg L-1 IBA, whereas, ‘Bargchenari’ and ‘Dehdez’ produced the highest rooting percentage and the root number per shoot at concentrations of 1000 and 1500 mg L-1 IBA respectively. Three months after transferring rooted shoots to soil mixtures, ‘Bargchenari’ genotype showed the highest survival rate (80%), shoot length (2.5 cm) and leaf number (4.65 leaves per shoot). In relation to survival percentage, the best medium was perlite, but plantlets showed better growth on perlite + peat medium.
Keywords
Ficus carica; IBA; regenerated shoot; rooting; soil mixture
Hrčak ID:
193534
URI
Publication date:
28.12.2017.
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